Prevalence of MDR and XDR Pseudomonas aeruginosa and Acinetobacter baumannii isolated in clinical samples from Children hospital

Abstract

Background: Acinetobacter baumannii and Pseudomonas aeruginosa are two major causative agents of nosocomial infections leading to the increase in morbidity and mortality rate globally, due to being MDR and XDR.

Objective: The aim of this study is to check the prevalence of MDR and XDR Acinetobacter baumannii and Pseudomonas aeruginosa phenotypes in hospital based samples.

Methods: Isolation of bacteria from various clinical samples is done using MacCokney agar and then identified using biochemicals. MDR and XDR strains were determined by antibiotic susceptibility testing (Kirby bauer disc diffusion assay).

Results: Out of 3248 clinical samples, Acinetobacter baumannii and Pseudomonas aeruginosa strains were detected in 309(9.51%) samples. Antibiotic susceptibility testing indicated that (16.50%) and (15.53%) of the P. aeruginosa and (74.75%) and (73.13%) of the Acinetobacter baumannii isolates were screened as the MDR and XDR strains. In wound samples, behavioral intensive care unit (BICU) and restoration ward, the frequency of MDR isolates was, 222 (71.8%), 187 (60.5%) and 63 (20.4%). The frequency of XDR isolates in burns was 30 (9.70%), while in BICU was 187 (59.54%), and restoration 58(18.77%).

Conclusion: Considering high isolation rates of MDR and XDR of Acinetobacter baumannii and Pseudomonas aeruginosa, it is now necessary to adopt prevention criteria to get rid of of MDR and XDR Acinetobacter baumannii and Pseudomonas aeruginosa from hospital wards.